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Notice that dry malt extract will differ from liquid malt extract in its sugar content material when diluted weight for weight:
We quantified complete microbes applying universal bacterial primers [29] by qPCR in 114 Grownup fecal DNA samples which were analyzed for microbiota composition using Illumina MiSeq for 16S rRNA gene amplicon sequencing [14]. The qPCR threshold cycle (Ct) values had been transformed into the estimates of bacterial genomes present in 1 g of feces like a proxy of overall bacterial counts.
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